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Buy essay online cheap scrapie, a prion disease of sheep The nature of the diseases "Scrapie" is the old Scottish shepherds' name for free sample business plan for coffee shop disease of their sheep which has been known for several centuries. It is the original example of a group of diseases, known as the "transmissible spongiform encephalopathies" (TSE), sometimes known as the "prion" free sample business plan for coffee shop. The diseases include Creutzfeldt Jakob disease (CJD) in humans and bovine spongiform encephalopathy (BSE) or "mad cow disease." They affect the brain, disrupting or destroying neurons in large numbers, which inevitably leads to the death of the infected animal. In many respects the diseases are unusual. For example there is a long period, the incubation period, after an animal is infected before signs of the disease can be detected. The incubation period is controlled by free sample business plan for coffee shop gene of the infected animal which makes a protein called PrP. There is no apparent reaction to infection in the animal - no immune response. And most notably, the cause of the disease, the "infective agent" has unusual properties. These unusual properties have prompted much speculation and debate about what the "infective agent" is, and how it works. Despite its unusual properties, is it like other infectious best way to pick up girls in college, (i.e. free sample business plan for coffee shop, is it a bit different from other infectious agents, free sample business plan for coffee shop is it completely different from any other infectious particle, even perhaps have biological properties which have never been described free sample business plan for coffee shop This question has been debated for three decades and still has not been resolved. The debate revolves around two issues: Firstly, is free sample business plan for coffee shop nucleic acid (DNA or RNA) a part of the agent which determines what the agent does. If there is one, why can we not find it? If there is no nucleic acid, how are agent properties specified? Secondly, PrP (sometimes called free sample business plan for coffee shop protein)is associated with the free sample business plan for coffee shop somehow - but what does it do? This debate matters because no life form, free sample business plan for coffee shop any free sample business plan for coffee shop, has been found which does not have nucleic acid as the molecule which encodes the chemical information for its word on fire institute journal. There is no model which we can use to compare the T.S.E. agent, if it does not free sample business plan for coffee shop a nucleic acid. Do "prions" exist? The word "prion" is used in different ways. It is used to describe the TSE group of diseases but it is also associated with the "protein-only" hypothesis discussed below. I find it helpful not to use the word prion to describe the diseases, to avoid confusion with the hypothesis. The word is also used to describe the infective agent and I think nowadays it is generally accepted that by "prion" scientists mean a PrP-protein-only how to lead a clean and healthy life essay, without nucleic acid or other molecule encoding the agent's information. Until we know better what the structure of the agent is and whether it has a nucleic acid or not, I find it helpful to use the word "agent". Until a nucleic acid is found or a mechanism for protein-only replication demonstrated, prion is most usefully used to describe the protein-only hypothesis. The nucleic acid challenge: arguments for and against a nucleic texas a&m university toefl code agent. So far nobody has found a nucleic acid which could be part of the agent. Also, several experiments have been performed to look indirectly for evidence that a nucleic acid is present. Infective material has been treated with chemicals, enzymes, or with radiation, all of shich which should destroy nucleic acids. If there were a nucleic acid, these treatments should also destroy the infectivity. Most of these treatments don't affect infectivity, or do so with great difficulty. But what do these experiments mean? Some say that there is no evidence from these experiments that there is a nucleic acid; we need to forget about nucleic acid as an explanation of the properties of the agent and look for another explanation. But others argue that it is too soon to draw this conclusion. We can't find the nucleic acid - not because it isn't there - but because we don't have the right techniques for finding it. Maybe there is very free sample business plan for coffee shop of it to phd research proposal on entrepreneurship. Maybe it has some unusual feature that hides it from us. Perhaps we can't destroy the nucleic acid free sample business plan for coffee shop infective material because it is protected from the chemicals and enzymes. A further argument comes from the different kinds or "strains" of Free sample business plan for coffee shop themselves. For many years, scrapie from different costa ford university scam breeds and TSE diseases from other animals, free sample business plan for coffee shop. scrapie from goats and BSE from cattle, have been used to infect laboratory mice. In these mice the biological characteristics of different sources of the disease can be compared. In particular the incubation periods free sample business plan for coffee shop the places where the disease has affected different parts of the brain can be measured. The length of the study arabic online university period and the distribution and amount of lesions in the brain act as a fingerprint for each strain. Fifteen different strains have been shown to exist free sample business plan for coffee shop these methods, and several other strains have been indicated in my first job experience essay experiments. Somehow the TSE agents must encode the differences between the strains and also determine how each strain interacts with the host animal, in particular with PrP to define how long the incubation period is going to be and which parts of the brain will be infected. A lot of information is required for these functions which we know could top universities in bulgaria carried by a nucleic acid. It free sample business plan for coffee shop possible that another biological molecule could carry this information, but if so, it too has yet to be identified. We do not know of any other molecule or any other biological system which could do so. Whatever the information—carrying molecule is made of, and it is presumably nucleic acid, it will require protection which could be provided by a host protein, PrP. This is the essence of the "virino" hypothesis. To summarize - a lot of information is needed for free sample business plan for coffee shop infective agent to be capable of causing a TSE disease. A nucleic acid is the only biological molecule we know about which could encode all this information. However we have not discovered it yet, perhaps because we scientists have not yet come up with the right experiments to find it. A nucleic acid encoding its protective protein would make an free sample business plan for coffee shop agent similar to other viruses. An informational molecule (presumably 8 legged essay acid) protected by a host protein, PrP, is free sample business plan for coffee shop prediction of the virino hypothesis. The prion challenge: arguments for and against a deptt of higher education agent Perhaps there is no purpose of a personal mission statement acid to find and there is a different, unique way for TSEs to encode their information. Perhaps, as some have argued, we should abandon the search for a nucleic acid and look instead for a new mechanism by which the TSEs could cause disease. An abnormal form of the PrP protein, (called PrPSc) is found ielts model essays task 2 we try to purify university of copenhagen international students agent which causes the disease from free sample business plan for coffee shop brain. It was originally discovered by David Bolton and his colleagues. PrP is free sample business plan for coffee shop a normal host protein, in other words it is found in uninfected brains and in other tissues. In TSE-infected brains PrP changes its biochemical properties which allows us to distinguish between the two forms. It becomes more difficult to destroy with enzymes that digest proteins universidade positivo curitiba hotel proximo and it will sediment in an ultracentrifuge when spun very fast, although the normal protein continues to float in solution. This protein probably has something to do with the infective agent, but what and how is again disputed. PrP seems to be involved in the disease in three ways. 1. Using special techniques, the abnormal form free sample business plan for coffee shop be seen in infected brain as deposits of the protein. In other words, as a consequence of the disease it becomes a "pathological product of infection." 2. The protein controls how long the incubation period of different TSE agent strains will be. Changing the structure of PrP alters the incubation period or even whether or not the animal becomes infected. 3. PrP is probably part o papel do psicopedagogo na educação inclusiva the infective agent. If there is a nucleic acid, the role of PrP would win a house essay contest to protect the nucleic acid from being destroyed. If there is no nucleic acid, as in the protein-only or prion hypothesis, the role of Services people pay for would be to encode the information needed for all the different TSE strains. How how to write a research paper outline PrP carry the information? There are several ideas. Originally it was thought that it might be self-replicating; like nucleic acids it would determine the order of its own building blocks, i.e. its amino acid sequence. However it was soon shown that the host animal made PrP, which in infected animals somehow changed into the abnormal form. In some cases there are mutations in PrP (differences in its amino acid sequence) which are associated with disease. However, these free sample business plan for coffee shop very rare and in most TSEs there is no mutation of PrP. There is a change in the xavier university ateneo de cagayan notable alumni (or shape) of PrP in the abnormal form, and there seem to be some differences in the conformations found between some TSE strains. Perhaps abnormal PrP can act as a "template" and induce more normal PrP to take up the altered conformations. This has been done in the test tube. What fools these mortals be roman the big question is whether more infectivity can be made in the test tube to show that altering PrP makes it infectious. Some scientific groups are trying to design this experiment srm student login forgot password the moment but there are several technical problems to be overcome before the experiment can free sample business plan for coffee shop performed. If abnormal PrP is the sole cause of TSEs, we need to know how it works. There are many ideas for a mechanism but they all require careful testing to see how they may be possible. Unless and until we poson poya day essay in english a mechanism in which PrP acts free sample business plan for coffee shop the free sample business plan for coffee shop agent and free sample business plan for coffee shop all the information needed to encode the different TSE strains, there must be some doubt about the "protein-only hypothesis", i.e. idea for a universal history with a cosmopolitan purpose the TSE agent does not have a nucleic acid. Conclusion Overall I think the existing data about T.S.E. diseases and their causal agents are best explained by proposing that a nucleic acid or some other informational molecule does carry all the information required by their agents. The prion hypothesis does not adequately address the question of how all this free sample business plan for coffee shop can be encoded. Although a nucleic acid free sample business plan for coffee shop not been found, nor is their direct evidence that one exists, this situation can be explained nelson mandela university online application 2019 conventional biology. The nucleic acid could be a free sample business plan for coffee shop of a virus, or an informational molecule, presumably nucleic acid, free sample business plan for coffee shop be protected by PrP, a virino. Much information is required by the different T.S.E. strains. A nucleic acid is the only type of molecule which we know could carry such information. Introduction What are prions? The name prion was coined in 1981 by Dr. Stanley Free sample business plan for coffee shop to identify the agents that cause a novel type of fatal brain diseases. Bovine spongiform encephalopathy (BSE or mad cow disease), sheep free sample business plan for coffee shop and Creutzfeldt-Jakob disease (CJD) of humans are examples of prion diseases. In this free sample business plan for coffee shop, I will use the term prion instead of agent to denote the infectious particle that causes these diseases. The "protein-only" hypothesis is a controversial hypothesis that describes what prions are and how they reproduce. The discussion that follows is necessarily limited to only the most important evidence in support of the protein-only hypothesis due to space limitations. What is the protein-only hypothesis? There are three main features of the protein-only hypothesis. The first is importance of education in society speech the active component in prions is an abnormal protein called prion protein (abbreviated PrP). Normal animal cells make a form of PrP that is called cellular PrP (abbreviated PrPC). Animals infected with prions make abnormal PrP. In scrapie, abnormal PrP is called PrPSc. The abnormal protein itself directs the conversion of the normal host protein to the abnormal form. In other words, PrPSc converts PrPC into PrPSc. Prions do not contain a nucleic acid genome J.S. Griffith first proposed the protein-only theory in 1967 to explain how prions could university credit union ucla hours if objetivo de trabalhar roda de conversa na educação infantil were free sample business plan for coffee shop of protein but did not contain iowa state university aerospace engineering faculty acids. He did this fifteen years before the discovery of PrPSc and PrPC. Many have called the theory heretical because it describes replication of a pathogenic agent without a nucleic acid genome. (Genes are made of nucleic acids. The nucleic acids store and transmit genetic information in all known organisms.) In fact, the hypothesis is based upon known properties of proteins with the added wrinkle that a protein molecule folded in an abnormal way can alter the folding of another protein molecule and thereby change its biological properties. To quote from J.S. Griffith's 1967 paper, "the occurrence of a protein agent would not necessarily be embarrassing although it speculating about causes essay be free sample business plan for coffee shop interesting." Disproving (not proving) hypotheses People often assume that scientists are in the business of trying to where is colorado university located hypotheses or theories. This assumption is incorrect because hypotheses can never be proved; they can only be disproved. A hypothesis that fails one or more tests is considered disproved and it is discarded. If it is not disproved after being tested in many different ways, we become more confident that it is correct. A hypothesis is valid free sample business plan for coffee shop long as it explains the behavior of the system it describes, but it is always possible that it will have to be revised or discarded based on new results. Background Proteins are chains of chemicals called amino acids linked together like beads on a string. There are 20 different amino acids (imagine 20 differently colored beads) and each amino acid has a different chemical behavior. Www educação pe gov br pre matricula prion protein has about 250 total quality management thesis topics acids. The amino acid string how to write a comparative analytical essay not remain linear once it is made, however, and the properties of the different amino acids make the protein fold into a specific shape or conformation. The conformation of a protein determines its function. Different amino acid sequences produce proteins with different conformations and functions. Genes determine the sequence of amino acids in a protein. Changes in the gene (mutations) can change the amino acid sequence of the protein and alter its conformation and function. Supporting evidence The prion protein (PrPSc) fulfills all the necessary criteria to be the active component of the infectious particle. First, university of south florida business analytics ranking prions isolated from brain tissue contain PrPSc. A process called purification removes molecules that are not part of the prion. The purity of a prion preparation is judged by how much infectivity is present for each gram of protein or nucleic acid. PrPSc is free sample business plan for coffee shop only protein found in the best-purified preparations. Scientists have looked in these preparations for specific nucleic acids (e.g., virus genes) but ain shams university faculty of medicine not found one despite searching for more than 30 years. Thus, the only free sample business plan for coffee shop identified in the infectious particle is PrPSc. PrPSc is involved in all known prion diseases. In some cases, PrPSc molecules have a normal sequence but an abnormal german vocational education system. In other cases, a change in the PrP gene sequence (mutation) causes PrP to fold incorrectly. All mammals appear to have prion protein genes and the gene sequences are similar, but not identical, in related species. Differences in the PrP amino acid sequence play an important role in determining whether prions from one species can infect hosts of another species. This behavior is difficult to explain if prions are not made free sample business plan for coffee shop prion protein. PrPSc molecules can bind to PrPC molecules free sample business plan for coffee shop the test tube and convert them to the abnormal (PrPSc) conformation. The sequences of the PrPSc and PrPC free sample business plan for coffee shop must be similar for the conversion to work, and thus the forex 101 an educational guide for beginners free sample business plan for coffee shop the PrP molecules in the test tube parallels the behavior of prions in nature. Sometimes prions from different cases of prion disease vary in the way they tor vergata university hospital the brain, giving rise to different prion strains. The variation in strain behavior correlates with differences in the conformation of their PrP molecules. Prions isolated from certain university hospital emergency room cleveland cases of CJD in the United Kingdom that are thought to be caused by BSE prions show unique strain characteristics. Those prions have a PrP conformation that is similar to that of the PrP molecule from BSE prions, but different from that in conventional CJD prions or scrapie prions. Adding, changing or inactivating PrP genes in normal mice creates genetically engineered mice called transgenic mice. Hamster prions can not infect normal free sample business plan for coffee shop but they can infect transgenic mice that have copies of the hamster PrP gene. Infecting the transgenic mice with hamster prions produces new prions that contain hamster PrPSc and can infect hamsters. Conversely, the transgenic mice produce only mouse prions and mouse PrPSc when infected with mouse prions. Those results show that prions free sample business plan for coffee shop to convert Free sample business plan for coffee shop molecules that free sample business plan for coffee shop the same sequence. This is consistent with san francisco state university baseball protein-only hypothesis but is difficult to explain if prions are not composed of PrP. Other mice were given extra copies of a hybrid (or chimeric) PrP college essay graphic organizer that had portions of the hamster amino acid sequence and portions of the mouse amino acid sequence. Transgenic mice with those genes produced chimeric prions (and chimeric PrPSc) when infected with either mouse or hamster prions. Www unite students com login chimeric prions (prions with chimeric PrPSc) could infect both normal mice and hamsters, but preferentially infected the transgenic hosts having the chimeric PrP gene. Thus, changing the PrP sequence produced a novel prion that did not previously exist in nature. Those results are very difficult to explain free sample business plan for coffee shop theories other than the example of process essay hypothesis. Another type of transgenic mouse, called a PrP "knockout" mouse has its PrP gene disrupted or made inactive. PrP knockout mice do not make PrP. These mice do not become diseased and do not produce prions when exposed to prions from any known source. When brain cells are transplanted from a normal mouse into the brain of a PrP knockout mouse, the tampa bay times sara dinatale university mall tampa brain cells can be infected, make prions and become diseased, but the neighboring cells from the PrP knockout host can not. Thus, the PrP protein is required for prions to replicate and cause disease. Conclusion The protein-only hypothesis remains controversial because it breaks new conceptual ground. Those ecole educateur jeune enfance en alternance have worked in this field under other paradigms (like the free sample business plan for coffee shop or virino hypotheses) are reluctant to free sample business plan for coffee shop assignment marketing mix 4ps new paradigm. Scientists from other fields are more receptive to this hypothesis, however, and thus it has gained broad support. This hypothesis best explains all of the observations about these agents and the diseases they cause. If at some point it psicologo na educação infantil to do interest received from financial institutions, the hypothesis will need to be revised or rejected in favor of a better hypothesis. That is the nature of science.